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. 2012 Mar 28;103(6):1136–1144. doi: 10.1111/j.1349-7006.2012.02243.x

Figure 2.

Figure 2

Overexpression of ADP‐ribosylation factor 1 (ARF1) mRNA in gastric carcinoma. The ARF1 gene is overexpressed in gastric carcinoma, as determined with quantitative RT‐PCR. (A) Quantitative RT‐PCR results. Levels of ARF1 mRNA were examined in 55 paired tumor (T) and adjacent normal (N) tissues. (B) Comparison of ARF1 mRNA expression in the advanced stages (III/IV) and early stages (I/II) of gastric carcinoma. (C) Western blot analysis of the 20‐kDa ARF1 protein. An equal amount (60 μg) of protein was loaded for each specimen and Coomassie blue stain was used as a loading control. The blots were exposed to film for ~3 min for stages I/II, and ~30 s for stages III/IV. (D) Immunohistochemical staining of ARF1 in two types of gastric carcinomas including intestinal (G02, G29) and diffuse type (G86, G95, G43, G99, G44, G40), stages I–IV. Positive staining of ARF1 is indicated by a dark‐brown color. ADP‐ribosylation factor 1 was observed mainly in the gastric cancer cells and barely in stromal cells.