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Proposed mechanism for biliverdin reduction by Rv2074. Proton donation to N22 on pyrrole ring B of biliverdin‐IXα by a hydroxonium ion generated by a nearby arginine residue occurs first, creating a cationic intermediate. This is then followed by hydride transfer from C5 of F420H2 to C10 of the intermediate, resulting in the formation of bilirubin‐IXα.
