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. 2017 Mar 6;7:43945. doi: 10.1038/srep43945

Figure 3. Characterization of RNAi vectors for Nr1d1.

Figure 3

(A) pCAG-Myc-Nr1d1 or (B) pCAG-Myc-Nr1d2 was co-transfected into COS7 cells with control pSUPER vector, pSUPER-mNR1D1#1 - #3. After 48 h, cells were harvested and subjected to western blotting (20 μg protein per lane) with anti-Myc. Anti-Sept11 was used for a loading control. (C) pCAG-Myc vector (−), pCAG-Myc-Nr1d1 (wt) or –Nr1d1-R (R) was co-transfected into COS7 cells with control pSUPER vector (−) or pSUPER-mNR1D1#1 (wt, R). Analyses were done as in (A).