Figure 4. Muc4 mRNA expression in Gal-3 null mutant (gal-3^−/−) and wild type (WT) C57BL/6 mice.

(a) Total mRNAs were extracted from jejunum (WT n = 9 (mice), gal-3^−/− n = 12) and lung (WT n = 14, gal-3^−/− n = 12). Muc4 and Gapdh (internal control) transcripts were quantified by RT-qPCR using the 2^−ΔΔCt method. Muc4 mRNA levels was arbitrarily set at 1 in WT mice and results are expressed as fold variation in gal-3^−/− vs WT mice. ***p < 0.001; NS, not significant by Student’s t-test. (b) Analyses of hnRNP-L expression by immunohistochemistry in jejunum and lung tissues from WT and gal-3^−/− mice, n = 4 mice in each condition.