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. 2017 Mar 6;7:43397. doi: 10.1038/srep43397

Figure 3. bHLH142 overexpressing anthers are defective in septum and stomium degeneration.

Figure 3

(a) Transverse sections of pre-anthesis staged WT and transgenic anthers (L42) undergoing dehiscence. Green and red arrows indicate intact septum and stomium in transgenic anthers, respectively. (b) WT and transgenic anthers analyzed under UV light to visualize lignin autofluorescence of endothecium regions. Red and yellow arrows indicate presence and absence secondary thickening of endothecium, respectively. (c) qRT-PCR expression data of anther dehiscence-related protein genes. Error bars indicate standard deviation (SD). The data are presented as the mean ± SD (n = 3). Asterisks indicate significant difference with respect to WT (‘***’ indicates t-test p-value ≤ 0.001, while ‘**’ indicates p-value ≤ 0.01). WT, Wild Type. Scales: A, 50 μm; B, 100 μm.