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. 2017 Mar 6;7:43397. doi: 10.1038/srep43397

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Venn diagram showing common and unique genes differentially regulated in tetrad and MP anther. (b) Histogram showing number of genes upregulated and downregulated in tetrad and MP stage of transgenic anthers compared to WT. Heat map showing log₂ fold changed in expression of cell wall modification (c), ROS signaling (d) and cell death (e)-related genes. (f) Validation of changed in expression of different metabolism-related genes through qRT-PCR. The data are presented as the mean ± SD (n = 3). Asterisks indicate significant difference with respect to WT (‘***’ indicates t-test p-value ≤ 0.001, ‘**’ p-value ≤ 0.01 and ‘*’ p-value ≤ 0.05).