| Plasmids |
| pRS425 |
LEU2, a multi-copy plasmid |
| pPS425-mXYL1-XYL2-XKS1-XYL1
|
Expression of XR (R276H), XDH, XK and XR through pPS425 |
| YCplac33 |
URA3, a single-copy plasmid |
| YCplac33-cbbM-sPRK
|
Expression of cbbM and sPRK through YCplac33 |
| YCplac33-cbbM-cfxP1
|
Expression of cbbL1-cbbS1 and PRK through YCplac33 |
| YCplac33-cbbM-sPRK-GroEL-GroES
|
Expression of cbbM, sPRK and GroEL-GroES through YCplac33 |
| YCplac33-cbbL1-cbbS1-cfxP1-HSP60-HSP10
|
Expression of cbbL1-cbbS1, PRK and HSP60-HSP10 through YCplac33 |
Strains
|
| YS58 |
MATa, leu2, his3,ura3, trp1 |
| YSX4 |
YS58 harboring pPS425-mXYL1-XYL2-XKS1-XYL1
|
| YSC000 |
YS58 harboring YCplac33 |
| YSC110 |
YS58 harboring YCplac33-cbbM-sPRK
|
| YSC111 |
YS58 harboring YCplac33-cbbM-sPRK-GroEL-GroES
|
| YSC220 |
YS58 harboring YCplac33-cbbM-cfxP1
|
| YSC222 |
YS58 harboring YCplac33-cbbM-cfxP1- HSP60-HSP10
|
| YSX4C000 |
YS58 harboring pPS425-mXYL1-XYL2-XKS1-XYL1 and YCplac33 |
| YSX4C110 |
YS58 harboring pPS425-mXYL1-XYL2-XKS1-XYL1 and YCplac33-cbbM-sPRK
|
| YSX4C111 |
YS58 harboring pPS425-mXYL1-XYL2-XKS1-XYL1 and YCplac33-cbbM-sPRK-GroEL-GroES
|
| YSX4C220 |
YS58 harboring pPS425-mXYL1-XYL2-XKS1-XYL1 and YCplac33-cbbL1-cbbS1-cfxP1
|
| YSX4C222 |
YS58 harboring pPS425-mXYL1-XYL2-XKS1-XYL1 and YCplac33-cbbL1-cbbS1-cfxP1-HSP60-HSP10
|
