. 2017 Mar 6;7:43847. doi: 10.1038/srep43847

Table 2. Candidate genes and associated primers for each pathway.

Gene ID	Pathway	Aedes gene name	Direction	Sequence (5′-3′)	Tm	Gene function
AAEL017251	RNAi	argonaute-2	Fw	ACAACAGCAACAATCCCAGA	60	Catalytic compound of RISC, mRNA cleavage
AAEL017251	RNAi	argonaute-2	Rv	GTGGACGTTGATCTTGTTGG	60	Catalytic compound of RISC, mRNA cleavage
AAEL002286	Autophagy	APG5	Fw	CCAGGACTTGTTGGAGGACT	56	Autophagosome elongation
AAEL002286	Autophagy	APG5	Rv	GTCCGGATAGCTGAGGTGTT	56	Autophagosome elongation
AAEL000627	Toll	cecropin-A	Fw	CCATGGCTGTTCTTCTCCTGA	60	Antimicrobial peptide
AAEL000627	Toll	cecropin-A	Rv	GGCGGCATTGAAAACTCGTT	60	Antimicrobial peptide
AAEL004833	Imd	diptericin-A	Fw	CCAATTCAGGAAGTGGAACC	56	Antimicrobial peptide
AAEL004833	Imd	diptericin-A	Rv	TGTTGATGGGTAGCTCCAAA	56	Antimicrobial peptide
AAEL014148	Imd	dredd	Fw	GTGGCTGTTATGCGAGAAGA	60	Initiatior caspase, cleavage of REL2
AAEL014148	Imd	dredd	Rv	AGCGTAGTTCTGCCTGAGGT	60	Initiatior caspase, cleavage of REL2
AAEL001932	Imd	FADD	Fw	GGGACCGTCGAACACTTCTT	60	Imd signal transducer
AAEL001932	Imd	FADD	Rv	CACTCAGCTGCATTAACCGC	60	Imd signal transducer
AAEL007768	Toll	MyD88	Fw	GGACTACAAGCGCTCGAACA	60	TOLL signaling cascade starter
AAEL007768	Toll	MyD88	Rv	CTGGTTTGGTTTGCGTTCGA	60	TOLL signaling cascade starter
AAEL006571	Toll	PELLE	Fw	ACAACCGACGAAAACTCCGA	56	Signaling molecule - Kinase
AAEL006571	Toll	PELLE	Rv	GCGAAGTTCTTCCCCACTGA	56	Signaling molecule - Kinase
AAEL000718	JAK/STAT	vir-1	Fw	GCCAAAGTCCGGTATTCTTC	60	Antiviral effector
AAEL000718	JAK/STAT	vir-1	Rv	TTCACGAGATCGTCAAGGTAA	60	Antiviral effector
AAEL004175	—	rpS17	Fw	TCCGTGGTATCTCCATCAAGCT	60	Ribosomal small subunit assembly
AAEL004175	—	rpS17	Rv	CACTTCCGGCACGTAGTTGTC	60	Ribosomal small subunit assembly
AE017196	—	WD0513	Fw	GTATCCAACAGATCTAAGC	60
AE017196	—	WD0513	Rv	ATAACCCTACTCATAGCTAG	60
NC_001474.2	—	DENV	Fw	AAGGACTAGAGGTTAGAGGAGACCC	60
			Rv	CGTTCTGTGCCTGGAATGATG	60
			Pr	HEX-AACAGCATATTGACGCTGGGAGAGACCAGA-BHQ1

Gene function from Flybase and Swiss-Prot.