Figure 2.

(A) Dwell-time mapping enables the absolute discrimination of SNV vs WT DNA. Note that the non-nucleic acid background molecules in the two groups were removed using the universal threshold (Supplementary Fig. S1). Inset: the average bound and unbound time of SNV and WT DNA. (B) The specificity and sensitivity of the assay for KRAS c.34 A mutation detection at varying values of the bound-state lifetime threshold. The threshold for 100% specificity is 6.1 s referring from (A). A more stringent threshold was set to 6.8 s by considering the derivation of the maximal t_bound of WT, threshold ≥ t_bound_max + 3 × std (t_bound_max).