Fig. S6.

Purity of metabolic tracers. (A) Purity of [1,6-¹³C2]-d-glucose. The metabolic tracer [1,6-¹³C2]-d-glucose was dissolved in DMSO-D6 at 5.6 mM and was measured by NMR as described in Materials and Methods. No any contamination of [2-¹³C]-acetate was found in [1,6-¹³C2]-d-glucose. (B) Purity of [3-¹³C]-pyruvate. The metabolic tracer [3-¹³C]-pyruvate was dissolved in DMSO-D6 at 1.0 mM and was measured by NMR as described. Trace amounts of [2-¹³C]-acetate were identified in [3-¹³C]-pyruvate (two insignificant peaks at 1.80 and 1.98 ppm).