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PCR primers used to amplify genomic regions of guide RNA targets
| Guide RNA target region | PCR primers |
| ACLY exon 3 | ACLY Ex3_F: GGATGGTTAGGGCTGGCTTTG |
| ACLY Ex3_R: GCTGGGAATGGGCATCAAGAC | |
| ACSS2 exon 1 | ACSS2 Ex1_F: GGAACTTGACGTGATGGGGCT |
| ACSS2 Ex1_R: CTCCCATGGAACCCTCGTCTC | |
| ACSS2 exon 2 | ACSS2 Ex2_F: GACCCAGGTGGTGGGTCCTA |
| ACSS2 Ex2_R: TGACACTTTGCTTACTCTACTGGGT |
