Skip to main content
. 2017 Feb 7;114(8):E1500–E1508. doi: 10.1073/pnas.1612991114

Fig. 7.

Fig. 7.

SGK3 retains ERα expression through maintaining EnR homeostasis via SERCA2b. (A) Western blotting analysis of EXE-R and LET-R cells after being treated with different concentrations of TG, as indicated, for 48 h. (B) Western blotting analysis of EXE-R and LET-R cells after being treated with 0.1 μM TG for different time, as indicated. (C) Western blotting analysis of EXE-R and LET-R cells after being transfected with siRNA negative control or SERCA2b siRNA for 72 h. (D) Western blotting analysis of LET-R and LET-R/SERCA2b-HA cells after being treated with different concentrations of GSK650394, as indicated, for 48 h. (E) Western blotting analysis of EXE-R and LET-R cells after being treated with 1 μM TG, 5 μg/mL brefeldin A (BFA), and 4 μg/mL tunicamycin (TM) for 48 h. (F) Schematic diagram of the three arms of EnR stress response or UPR. (G) Western blotting analysis of MCF7aro cells treated with 0.5 μM TG alone or plus 1 μM GSK2656157 (PERK inhibitor) or 25 μM 4μ8C (IRE1 inhibitor) or two in combination for 24 h. (H) Western blotting analysis of MCF7aro cells treated with 2.5 μg/mL BFA alone or plus 1 μM GSK2656157 (PERK inhibitor) or 25 μM 4μ8C (IRE1 inhibitor) or two in combination for 24 h. (I) Western blotting analysis of EXE-R cells and LET-R cells after being treated with 20 μM GSK650394, 2 μM PERK inhibitor (GSK2656157), alone or in combination for 24 h. (J) Western blotting analysis of MCF7aro cells treated with different concentrations of GSK650394, as indicated, for 48 h. (K) Western blotting analysis of MCF7aro cells transfected with siRNA negative control or SGK3 siRNA for 72 h.