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. 2017 Feb 6;114(8):1916–1921. doi: 10.1073/pnas.1610192114

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Fig. 3. — TROSY-HSQC analysis revealed chemical-shift perturbations in IκBα AR3, AR5, and AR6 upon DNA binding to the NFκB–IκBα(5xPEST) complex. (A) Overlaid TROSY-HSQC spectra of the binary NFκB–²H¹⁵N IκBα(5xPEST) complex and the ternary NFκB–DNA–²H¹⁵N IκBα(5xPEST) complex. (B) Chemical-shift perturbations as a function of residue number. Residues for which DNA binding resulted in a chemical-shift perturbation of more than one SD from the mean were Q165 (AR3), T219 (AR5), R245 (AR5), V246 (AR5), L256 (AR6), T257 (AR6), Q266 (AR6), and E284Q (PEST). Resonances that disappeared upon DNA binding were E128 (AR2), R140 (AR3), N182 (AR4), D226 (AR5), Y251 (AR6), S252 (AR6), W258 (AR6), G270 (AR6), L272 (AR6), and E275 (AR6). The insets show a selection of peaks corresponding to each AR.