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. 2017 Jan 30;114(8):E1395–E1404. doi: 10.1073/pnas.1621188114

Fig. 2.

Fig. 2.

IRE1 regulates lipid-induced IL-1β secretion in mouse and human macrophages. Il-1β (A) mRNA and (B) protein levels measured from LPS-primed and PA-stimulated mouse BMDMs treated with 150 μM STF-083010 or DMSO (control) by qRT-PCR and ELISA, respectively. Il-1β (C) mRNA and (D) protein levels measured from LPS-primed and PA-stimulated mouse BMDMs transfected with siRNAs against Ire1α or Xbp1 by qRT-PCR and ELISA, respectively. (E) Secreted IL-1β from LPS-primed, PA-stimulated human PBMCs treated or not treated with 100 μM 4μ8c measured by ELISA. (F) Same as E but protein levels were measured by immunoblotting to show the immature and processed forms of the cytokine (representative image of three independent blots). Statistics are the same as in Fig. 1. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.