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. 2017 Jan 30;114(8):E1395–E1404. doi: 10.1073/pnas.1621188114

Fig. S3.

Fig. S3.

IRE1 regulates lipid-induced CCL2 production in macrophages. (A) qRT-PCR analysis of Ccl2 mRNA levels in LPS-primed, PA-stimulated BMDMs that were treated with 150 μM STF-083010 or DMSO (control). (B) ELISA measurements of secreted CCL2 from the conditioned medium used to culture the cells in A. (C and D) qRT-PCR analysis of Ccl2 mRNA levels in LPS-primed, PA-stimulated BMDMs that were (C) treated with 4µ8c (at the indicated doses) or DMSO (control) or (D) transfected with siRNAs against Ire1α or Xbp1. (E) ELISA measurements of secreted CCL2 from the cells in D. (F) qRT-PCR analysis of Ccl2 mRNA levels in LPS-primed, PA-stimulated human PBMCs treated with 4µ8c (at the indicated doses) or DMSO (control). Statistics are the same as in Fig. 1. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.