Fig. 4.

Comparing high-throughout analysis with individual analysis in vivo. (A) Workflow used to compare high-throughput nanoparticle analysis with traditional, individual analysis. Thirty nanoparticles with varying PEG characteristics were injected. Ten of the nanoparticles, with a range of liver biodistributions, were analyzed individually by formulating them with siRNA targeting factor 7 (F7), a gene expressed in hepatocytes. Mice were injected with one siRNA-containing nanoparticle at a siRNA dose of 0.10 mg/kg, and the resulting factor 7 protein knockdown was compared with the barcode liver data. (B) Factor 7 protein reduction, tested one nanoparticle at a time, plotted against normalized barcode delivery in the liver 15 min after i.v. injection, after 30 nanoparticles were tested simultaneously. Data are shown as average ± SD, and n = 3–4 mice/group.