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. 2017 Feb 7;114(8):1934–1939. doi: 10.1073/pnas.1621359114

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Fig. 2. — Bsk is essential for Hpo activation-induced cell invasion. Fluorescence micrographs of wing discs are shown, anterior is to the Left, and cells are labeled with GFP expression. (A–D) Depletion of yki by ptc-Gal4 up-regulates puc transcription (B) and JNK phosphorylation (D). (E–J) Expression of Bsk^DN completely suppressed loss of yki (E), expression of Wts (G) or Hpo (I) induced cell invasion and MMP1 expression (F, H, and J). (Magnification: 20×.) Genotypes: (A) ptc-Gal4 UAS-GFP/+; puc^E69/+; (B) ptc-Gal4 UAS-GFP/+; UAS-yki.RNAi, UAS-Dcr2/puc^E69; (C) ptc-Gal4 UAS-GFP/+; (D and E) ptc-Gal4 UAS-GFP/+; UAS-yki.RNAi, UAS-Dcr2/+; (F) ptc-Gal4 UAS-GFP/+; UAS-yki.RNAi, UAS-Dcr2/UAS-Bsk^DN; (G) ptc-Gal4 UAS-GFP/+; UAS-Wts/tub-Gal80^ts; (H) ptc-Gal4 UAS-GFP/+; UAS-Wts/UAS-Bsk^DN, tub-Gal80^ts; (I) ptc-Gal4 UAS-GFP/UAS-Hpo; tub-Gal80^ts/+; and (J) ptc-Gal4 UAS-GFP/UAS-Hpo; tub-Gal80^ts/UAS-Bsk^DN.