Fig. S6.

Ban is essential for Hpo-induced cell invasion. Fluorescence micrographs of wing discs are shown, anterior is to the Left, and cells are labeled with GFP (A and B) or dsRed (C and D). Ectopic Hippo expression induced cell invasion (A′) and MMP1 activation (A′′) were both dramatically suppressed by coexpression of Ban (B–B′′). Loss of ban induced puc activation (C) were completely suppressed by inhibiting JNK signaling (D). (E) Quantification of Fig. 4 F′, G, and G′. Results are shown as mean + SEM. P values were calculated using Student’s t test. ***P < 0.001. Genotypes: (A) ptc-Gal4 UAS-GFP/UAS-Hpo; tub-Gal80^ts/+. (B) ptc-Gal4 UAS-GFP/UAS-Hpo; tub-Gal80^ts/UAS-Ban. (C) dpp-Gal4, UAS-ban-sp/puc^E69. (D) dpp-Gal4, UAS-ban-sp/UAS-Puc, puc^E69. (Magnification: 20×.)