Fig. 2.

Gata4 is required for DMP development. The Hh-receiving cardiac fate map was abnormal in Gata4-mutant embryos. Hh-receiving cells were marked by β-gal expression in Gli1^Cre-ERT2/+;R26R^fl/+ embryos at E7.5 and E8.5 by TM administration and were analyzed by lacZ staining at E10.5. A marked hypoplastic or missing DMP was observed in Gli1^Cre-ERT2/+;Gata4^fl/+;R26R^fl/+ embryos (C, D, G, and H) compared with the Gli1^Cre-ERT2/+;R26R^fl/+ embryos (A, B, E, and F). C, D, G, and H show the boxed areas in A, B, E, and F, respectively, at higher magnification. (Magnification: 100× in A, B, E, and F; 400× in C, D, G, and H.)