Fig. 6.

Gata4 interacts with Hh signaling in AV septation. (A–E) Histology of Gata4 transgenic mouse embryo hearts at E14.5. Asterisks indicate missing structures of atrial septation. A′–E′ show the boxed areas in A–E at higher magnification. (Magnification: 40× in A–E; 100× in A′–E′.) (F) Gene expression in the pSHF of Gli1^Cre-ERT2/+;Gata4^fl/fl embryos was measured by real-time PCR and was compared with gene expression in Gata4^fl/+ embryos. (G) Gene expression in the pSHF of Smo^+/− or Smo^−/− embryos was measured by real-time PCR and was compared with the gene expression in wild-type embryos. (H) Schematic of the mouse Gli1 genomic locus including Gata4-binding regions and the cloned genomic fragments used for Gata4 regulation assays (luciferase reporter assay and ChIP-qPCR). (I) Gata4-stimulated firefly luciferase expression is seen in wild-type Gli1-Fr but not in mutated Gli1-Fr-M1 and Gli1-Fr-M2 fragments. Results are presented as mean ± SEM; n = 3; *P < 0.05 compared with pGL3. (J) Enrichment of Gata4-responsive Gli1 genomic fragments in the SHF by Gata4 ChIP-qPCR. Results are presented as mean ± SEM; n = 3; * P < 0.05. (K–M) Histology of Gata4 transgenic mouse embryo hearts at E14.5. Asterisks indicate missing structures of atrial septation. (Magnification 40× in K–M; 100× in K′–M′.)