Fig. S1.

Confirmation of tissue-specific knockdown of Gata4 by gel electrophoresis. (A) DNA was extracted from SHF and heart tissues of Gata4^fl/+;Gli1^Cre-ERT2/+ mice and was tested for Cre-mediated knockdown of Gata4. (B) DNA was extracted from tail and heart tissues of Gata4^fl/+;Tnt^Cre/+ mice and was tested for Cre-mediated knockdown of Gata4. (C) DNA was extracted from tail and heart tissues of Gata4^fl/+;Tie2^Cre/+ mice and was tested for Cre-mediated knockdown of Gata4. (D) DNA was extracted from SHF, heart, and tail of Gata4^fl/+;Mef2c^Cre/+ mice and was tested for Cre-mediated knockdown of Gata4. The following primers were used: Cre forward: 5′-TCGACCAGGTTCGTTC ACTCATGG-3′; Cre reverse: 5′-CAGGCTAAGTGCCTTCTCTACACC-3′; Gata4-WT/flox forward: 5′-ACCCTGGAAGAC ACCCCAATCTCGG-3′; Gata4-del forward: 5′-TGTCATTCTTCGCTGGAGCCGC-3′; Gata4 reverse: 5′-TCCATGAGAC CCCAGAGTGTGCCTGA-3′. The size of Cre product is ∼220 bp. The Gata4 wild type and Gata4-flox products are ∼510 bp and 530 bp in size, respectively. The size of the Gata4-del product is ∼300 bp.