Table 1.
Expression of selected genes in heart, aSHF, and pSHF of mouse embryos at E9.5 measured by RNA-seq (n = 6)
Gene | Heart | aSHF | pSHF |
Gata4 | 37.43 ± 6.31a | 4.00 ± 3.12b | 18.86 ± 4.09c |
Tbx5 | 24.77 ± 2.21a | 0.91 ± 0.76b | 12.28 ± 1.98c |
Fgf8 | 2.12 ± 1.16a | 6.94 ± 2.36b | 3.87 ± 1.76b |
Tbx1 | 0.39 ± 0.17a | 14.12 ± 1.64b | 9.20 ± 2.12c |
Gli1 | 1.32 ± 0.39a | 14.76 ± 4.52b | 16.26 ± 4.26b |
Isl1 | 3.71 ± 1.21a | 22.84 ± 3.78b | 17.44 ± 4.01b |
Pten | 13.06 ± 0.79a | 14.92 ± 1.16a | 14.88 ± 0.70a |
Adipoq | 0.18 ± 0.12a | 0.00 ± 0.02a | 0.02 ± 0.04a |
Mouse embryos were microdissected at E9.5 as described in Materials and Methods. The significant difference in the expression of each gene in heart, aSHF, and pSHF was tested by ANOVA analysis. Different letters denote a significant difference between different tissues (a vs. b, a vs. c, b vs. c; P < 0.05, n = 6). Adipoq, adiponectin.