Table 2.

Incidence of ASDs in Gata4-mutant embryos

Genotype	ASD	Total	Type	Vs. littermate control	P value
Conditional mutation of Gata4
Gata4+/−;EllaCre/+	7	18	All primum	vs. EIIacre/+ (0/13)	0.0053
Gata4fl/+;TntCre/+	0	12	—	vs. Gata4fl/+ (0/13)	1
Gata4fl/+; TieCre/+	0	23	—	vs. Gata4fl/+ (0/13)	1
Gata4fl/+;Gli1Cre-ERT2/+ (TM at E7.5 and E8.5)	13	21	All primum	vs. Gata4fl/+ (0/15)	<0.0001
Gata4fl/+;Gli1Cre-ERT2/+ (TM at E8.5 and E9.5)	2	9	All primum	vs. Gata4fl/+ (0/10)	0.0675
					0.1053*
Gata4fl/+; Mef2cAHF::Cre	1	22	All primum	vs. Gata4fl/+ (0/15)	1
Tbx5–Gata4 compound mutant embryos
Tbx5fl/+;Gata4fl/+; Mef2cAHF::Cre	3	9	1 primum	vs. Gata4fl/+;Mef2cAHF::Cre (0/13)	0.0125
			2 common atrium with CCAVC		0.0273*
				vs. Tbx5fl/+;Mef2cAHF::Cre (0/13)	0.0125
					0.0273*
Pten–Gata4 compound mutant embryos
Ptenfl/+;Gata4fl/+;Gli1Cre-ERT2/+	1	10	All primum	vs. Gata4fl/+;Gli1Cre-ERT2/+ (4/6)	0.0179
					0.0179*
				vs. Ptenfl/+;Gli1Cre-ERT2/+ (0/18)	0.1719
Smo – Gata4 compound mutant embryos
Smofl/+;Gata4fl/+; Mef2cAHF::Cre	5	15	All common atrium	vs. Gata4fl/+;Mef2cAHF::Cre (0/9)	0.0258
					0.0590*
				vs. Smofl/+;Mef2cAHF::Cre (0/12)	0.0134
					0.0235*
Gata4fl/+,SmoM2fl/+,Gli1Cre-ERT2/+	1	10	All primum	vs. SmoM2fl/+;Gli1Cre-ERT2/+ (0/7)	0.3719
				vs. Gata4fl/+;Gli1Cre-ERT2/+ (13/21)	0.0106
					0.0045*

ASDs were evaluated by the integrity and completion of the DMP and the AV cushion. Incidence of ASDs was evaluated in Gata4-mutant embryos at E13.5.

^*If the χ² P value was significant within the range (0.01 < P value < 0.1), a second P value was reported using the one-tailed Fisher exact test.