Table 3.

Genomic regions with Gata4-binding sites assessed by luciferase reporter assay and ChIP-qPCR

Gene name	Luciferase assay				ChIP
	Genomic fragment	Locus	Luciferase results	Gata4-binding sites in subcloned fragments	Genomic fragment	Locus	ChIP results, % of input
Gli1	Gli1-Fr	chr10:126775570–126776129	42.1 ± 16.2, P = 0.0478	chr10:126775655–126775660	Gli1-Fr1	chr10:126775576–126775736	0.44 ± 0.12, P = 0.0039
				chr10:126776103–126776108
	Gli-Fr-M1		6.3 ± 25.8, P = 0.4711	chr10:126776103–126776108*	Gli1-Fr2	chr10:126775984–126776129	0.09 ± 0.01, P = 0.0163
	Gli1-Fr-M2		−6.3 ± 22.3, P = 0.4270	chr10:126775655–126775660*	Negative control	chr10:126771190–126771322	0.01 ± 0.01

All genomic coordinates are shown in mouse genome build mm9.

^*This site was deleted in the mutated subclone of the Gata4 fragment.