Table S1.
List of strains used in this study
| Strain | Genotype | Source | Used to assay |
| DMA580 | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22:KanMX4 | Giaever et al., 2002 | N/A |
| YTZ3 | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 | This study | Morphology |
| YTZ44 | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::STE50IDR-S155A,S196A,S202A,T244A,S248A | This study | Morphology |
| YTZ45 | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::STE50 Cgla IDR | This study | Morphology |
| YTZ49 | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::STE50 Lklu IDR | This study | Morphology |
| YTZ8R | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 HOG1-Cterm::yemGFP pCAN1::pRPL39-ymCherry-LEU2 | This study | Hog1 signaling dynamics |
| YTZ8B | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 HOG1-Cterm::yemGFP | This study | Hog1 signaling dynamics |
| YTZ24R | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 HOG1-Cterm::yemGFP STE50IDR::STE50IDR-S155A,S196A S202AT244A,S248A pCAN1::pRPL39-ymCherry-LEU2 | This study | Hog1 signaling dynamics |
| YTZ24B | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 HOG1-Cterm::yemGFP STE50IDR::STE50IDR-S155A,S196A,S202AT244A,S248A pCAN1::pRPL39-mTagBFP2-LEU2 | This study | Hog1 signaling dynamics |
| YTZ25R | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 HOG1-Cterm::yemGFP STE50IDR::STE50 Cgla IDR pCAN1::pRPL39-ymCherry-LEU2 | This study | Hog1 signaling dynamics |
| YTZ25B | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 HOG1-Cterm::yemGFP STE50IDR::STE50 Cgla IDR pCAN1::pRPL39-mTagBFP2-LEU2 | This study | Hog1 signaling dynamics |
| YTZ29R | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 HOG1-Cterm::yemGFP STE50IDR::STE50 lKlu IDR pCAN1::pRPL39-ymCherry-LEU2 | This study | Hog1 signaling dynamics |
| YTZ29B | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 HOG1-Cterm::yemGFP STE50IDR::STE50 lKlu IDR pCAN1::pRPL39-mTagBFP2-LEU2 | This study | Hog1 signaling dynamics |
| YTZ60 | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 HO::pFUS1-yemGFP-klURA3 | This study | Fus3 basal signaling |
| YTZ62 | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::STE50IDR-S155A HO::pFUS1-yemGFP-klURA3 | This study | Fus3 basal signaling |
| YTZ62EE | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::STE50IDR- SP155-156EE HO::pFUS1-yemGFP-klURA3 | This study | Fus3 basal signaling |
| YTZ63 | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::STE50IDR-S155A,S196A,S202A HO::pFUS1-yemGFP-klURA3 | This study | Fus3 basal signaling |
| YTZ63EE | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::STE50IDR- SP155-156EE,SP196-197EE,SP202-203EE HO::pFUS1-yemGFP-klURA3 | This study | Fus3 basal signaling |
| YTZ64 | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::STE50IDR-S155A,S196A,S202AT244A,S248A HO::pFUS1-yemGFP-klURA3 | This study | Fus3 basal signaling |
| YTZ64EE | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::STE50IDR- SP155-156EE,SP196-197EE,SP202-203EE,TP244-245EE,SP248-249EE HO::pFUS1-yemGFP-klURA3 | This study | Fus3 basal signaling |
| YTZ65 | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::Cgla IDR HO::pFUS1-yemGFP-klURA3 | This study | Fus3 basal signaling |
| YTZ66 | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::Zrou IDR HO::pFUS1-yemGFP-klURA3 | This study | Fus3 basal signaling |
| YTZ67 | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::Lwal IDR HO::pFUS1-yemGFP-klURA3 | This study | Fus3 basal signaling |
| YTZ68 | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::Lthe IDR HO::pFUS1-yemGFP-klURA3 | This study | Fus3 basal signaling |
| YTZ69 | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::Lklu IDR HO::pFUS1-yemGFP-klURA3 | This study | Fus3 basal signaling |
| YTZ70 | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::Klac IDR HO::pFUS1-yemGFP-klURA3 | This study | Fus3 basal signaling |
| YTZ71 | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::Scer-Lklu IDR HO::pFUS1-yemGFP-klURA3 | This study | Fus3 basal signaling |
| YTZ72 | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::Scer-Zrou IDR HO::pFUS1-yemGFP-klURA3 | This study | Fus3 basal signaling |
| YTZ73 | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::Klac-Scer IDR HO::pFUS1-yemGFP-klURA3 | This study | Fus3 basal signaling |
| YTZ3R | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 pCAN1::pRPL39-ymCherry-LEU2 | This study | Fitness |
| YTZ3B | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 pCAN1::pRPL39-mTagBFP2-LEU2 | This study | Fitness |
| YTZ44R | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::STE50IDR-S155A,S196A,S202AT244A,S248A pCAN1::pRPL39-ymCherry-LEU2 | This study | Fitness |
| YTZ44B | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::STE50IDR-S155A,S196A,S202AT244A,S248A pCAN1::pRPL39-mTagBFP2-LEU2 | This study | Fitness |
| YTZ45R | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::STE50 Cgla IDR pCAN1::pRPL39-ymCherry-LEU2 | This study | Fitness |
| YTZ45B | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::STE50 Cgla IDR pCAN1::pRPL39-mTagBFP2-LEU2 | This study | Fitness |
| YTZ49R | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::STE50 Lklu IDR pCAN1::pRPL39-ymCherry-LEU2 | This study | Fitness |
| YTZ49B | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::STE50 Lklu IDR pCAN1::pRPL39-mTagBFP2-LEU2 | This study | Fitness |
| YTZ54 | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::STE50IDR-S155A,S196A,S202A,TP244-245EE,SP248-249EE | This study | Morphology |
| YTZ74 | MATa his3∆1 leu2∆0 ura3∆0 met15∆0 SSK22::HisMX3 SSK2∆0 STE50IDR::STE50IDR-S155A,S196A,S202A,TP244-245EE,SP248-249EE HO::pFUS1-yemGFP-klURA3 | This study | Fus3 basal signaling |