Fig. S5.
Potential energies used for the MC simulations' coordinates and the output free-energy curves calculated using the distribution. Note that hydrolysis of ATP and Pi release were normalized separately, even though they use a continuous coordinate (nucleotide-Pi distance), because the barrier of both reactions together was too high to obtain good sampling. The green and red curves represent the TL or the LL in the angle curves, and binding to the previous actin particle (i − 1) or next actin particle (i + 1) for the free head, respectively, where i is the index of the actin particle to which the other myosin head is attached.