Fig. 1.

Overview of solubility deep mutational scans for TEM-1.1 and LGK. (Left) Screens used in the present work. In YSD, the protein is exported to the surface and labeled by a fluorescent antibody that is specific for a C-terminal epitope tag. The top 5% of cells by fluorescence intensity are collected by FACS. For Tat export, a protein is fused to a C-terminal beta-lactamase that requires periplasm localization for activity. Variants are selected on plates containing high antibiotic concentrations. (Center and Right) Heat maps of solubility scores for selected residues of TEM-1.1 and LGK. Residues in the active site are indicated by (*), interface by (I), and proximal to the C terminus by (C).