Fig. 2.

Validation of solubility datasets. (A) Nonsense vs. missense solubility scores for YSD (LGK, TEM-1.1). (B) Fraction of beneficial mutations above the lower bounds versus contact number for LGK and TEM-1.1 (residues 61–215). Known stabilizing mutations (yellow) are mapped onto TEM-1.1 (C; PDB ID code 1M40) and LGK (D; PDB ID code 4ZLU). (Insets) Structural basis of the stabilizing mutations, shown as yellow sticks, along with the corresponding solubility scores identified by deep sequencing.