Fig. S2.

Iterative reduction of image size. To target the desired structure with a small scan, it is often helpful to increase the imaging resolution iteratively while reducing the image size. From Left to Right: Confocal overview scan (piezo driven) of a nucleus; confocal scan (electro-optic scanners) for drift correction; low-resolution STED scan (electro-optic scanners, 300 × 300-nm scan size) to identify individual NPCs; high-resolution STED image (electro-optic scanners, 100 × 100-nm scan size) reveals the substructure of a single NPC.