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STED imaging of Xenopus NPCs stained with Abberior Star Red-labeled anti-Nup98 nanobody TP377. (A) Comparison of images with different scan sizes. STED power: 336 mW. Cumulative pixel dwell time: 500 μs. Color scale: 0–50 (for all). (B) Comparison of images with different scan sizes. STED power: 147 mW. Cumulative pixel dwell time: 500 μs. Color scale: 0–80 (for all).
