Table S2.
Replication and transduction of CTX-1
| Primary transduction | Secondary transduction | |||||
| Donor | pCTX produced from the donor | Transduction recipient strain | Transduction efficiency | Transductants (transduced pCTX) | Transduction recipient strain | Transduction efficiency |
| PM14-U1 | pCTX-1kan-P* | O395 | 105 | O395 (pCTX-1kan-P) | O395 | 5 × 107 |
| MG116025 | 3 ×105 | |||||
| MG116025-toxTF139Y | 0 | |||||
| A213- toxTY139F | 105 | |||||
| IB4122- toxTY139F | 5 × 10 | |||||
| MG116025 | 102 | MG116025 (pCTX-1kan-P) | O395 | 5 × 106 | ||
| MG116025 | 103 | |||||
| MG116025-toxTF139Y | 0 | |||||
| A213- toxTY139F | 10 | |||||
| IB4122-toxTY139F | 0 | |||||
| MG116025-toxTF139Y | 0 | |||||
| A213- toxTY139F | 104 | A213-toxTY139F (pCTX-1kan-P) | O395 | 5 × 104 | ||
| MG116025 | 2 × 10 | |||||
| MG116025-toxTF139Y | 0 | |||||
| A213- toxTY139F | 2 × 102 | |||||
| IB4122- toxTY139F | 0 | |||||
| IB4122 toxTY139F | 102 | B4122 toxTY139F(pCTX-1kan-P) | ||||
| PM20 | pCTX-1kan-L† | O395 | 105 | O395 (pCTX-1kan-L) | O395 | 3 × 107 |
| MG116025 | 3 × 105 | |||||
| MG116025-toxTF139Y | 0 | |||||
| A213- toxTY139F | 106 | |||||
| IB4122- toxTY139F | 103 | |||||
| MG116025 | 102 | MG116025 (pCTX-1kan-L) | O395 | 5 × 104 | ||
| MG116025 | 5 × 10 | |||||
| MG116025-toxTF139Y | 0 | |||||
| A213- toxTY139F | 102 | |||||
| IB4122- toxTY139F | 10 | |||||
| MG116025-toxTF139Y | 0 | |||||
| A213- toxTY139F | 104 | A213- toxTY139F (pCTX-1kan-L) | O395 | 2 × 105 | ||
| MG116025 | 10 | |||||
| MG116025-toxTF139Y | 0 | |||||
| A213- toxTY139F | 102 | |||||
| IB4122- toxTY139F | 0 | |||||
| IB4122- toxTY139F | 10 | |||||
*
pCTX-1kan-P: pCTX-1kan produced from the plasmid pUC-CTX-1kan.
†
pCTX-1kan-L: pCTX-1kan produced from the lysogenic CTX prophage.