Table S3.
Replication and transduction of CTX-2
| Primary transduction | Secondary transduction | |||||
| Donor | pCTX produced from the donor | Transduction recipient strain | Transduction efficiency | Transductants (transduced pCTX) | Transduction recipient strain | Transduction efficiency |
| PM14-U2 | pCTX-2kan-P* | MG116025 | 2 × 10 | MG116025 (pCTX-2kan-P) | MG116025 | 2 × 104 |
| MG116025-toxTF139Y | 0 | |||||
| A213- toxTY139F | 3 × 103 | |||||
| IB4122 toxTY139F | 0 | |||||
| O395 | 0 | |||||
| MG116025-toxTF139Y | 0 | |||||
| A213- toxTY139F | 10 | |||||
| IB4122 toxTY139F | 0 | |||||
| O395 | 0 | |||||
| PM22 | pCTX-2kan-BL† | MG116025 | 5 × 103 | MG116025 (pCTX-2kan-BL) | MG116025 | 107 |
| MG116025-toxTF139Y | 10 | |||||
| A213- toxTY139F | 4 × 106 | |||||
| IB4122-toxTY139F | 102 | |||||
| O395 | 300‡ | |||||
| MG116025-toxTF139Y | 0 | |||||
| A213- toxTY139F | 5 × 102 | |||||
| IB4122- toxTY139F | 10 | |||||
| O395 | 0 | |||||
| PM9 | pCTX-2kan-VL§ | MG116025 | 103 | MG116025 (pCTX-2kan-VL) | MG116025 | 2 × 107 |
| MG116025-toxTF139Y | 0 | |||||
| A213- toxTY139F | 106 | |||||
| IB4122-toxTY139F | 1 × 103 | |||||
| O395 | 200* | |||||
| MG116025-toxTF139Y | 0 | |||||
| A213- toxTY139F | 103 | A213- toxTY139F (pCTX-2kan-VL) | MG116025 | 2 × 103 | ||
| MG116025-toxTF139Y | 0 | |||||
| A213- toxTY139F | 2 × 103 | |||||
| IB4122- toxTY139F | 0 | |||||
| O395 | 0 | |||||
| IB4122- toxTY139F | 10 | IB4122- toxTY139F (pCTX-2kan-VL) | ||||
| O395 | 5¶ | |||||
| PM10 | MG116025 | 0 | ||||
*
pCTX-2kan-P: pCTX-2kan produced from the plasmid pUC-CTX-2kan.
†
pCTX-2kan-BL: pCTX-2kan produced from lysogenic CTX-2 in PM22, which is a derivative of strain B33.
‡
O395 transductants were transduced by CTX-2kan (one-third of transductants) or CTX phage (two-thirds of transductants) that contained rstREl Tor generated by interstrand recombination between CTX-2kan and lysogenic CTX-1 in MG116025.
§
pCTX02kan-VL: pCTX-2kan produced from lysogenic CTX-2 in PM9, which is a derivative of strain V212-1.
¶
These five O395 transductants were transduced by CTX phage that contained rstREl Tor generated by interstrand recombination between CTX-2kan and lysogenic CTX-1 in V212-1 derivative PM9.