Table S4.
Primary transduction | Secondary transduction | |||||
Donor | pCTX produced from the donor | Transduction recipient strain | Transduction efficiency | Transductants (transduced pCTX) | Transduction recipient strain | Transduction efficiency |
PM14-U3 | pCTX-clakan-P* | MG116025 | 1 × 102 | MG116025 (pCTX-clakan-P) | MG116025 | 3 × 104 |
MG116025-toxTF139Y | 0 | |||||
A213- toxTY139F | 2 × 104 | |||||
IB4122- toxTY139F | 0 | |||||
O395 | 0 | |||||
MG116025-toxTF139Y | 0 | |||||
A213- toxTY139F | 4 × 10 | A213- toxTY139F (pCTX-clakan-P) | ||||
IB4122- toxTY139F | 3 × 10 | IB4122- toxTY139F (pCTX-clakan-P) | ||||
O395 | 0 |
pCTX-clakan-P: pCTX-clakan produced from the plasmid pUC-CTX-clakan.