Table 1.

Transduction efficiency of selected V. cholerae strains and plasmid-based replication

Strains	Description	Genetic structure		CTX phage produced	Transduction recipient	Transduction efficiency*
		Chromosome 1	Chromosome 2
PM20	N16961 derivative	TLC:CTX-1kan:RS1	No element	CTX-1kan-C1†	O395	105
					MG116025	102
PM14-U1	PM14 transformed with pUC-CTX-1kan	TLC:RS1	No element	CTX-1kan-P‡	O395	105
PM14-U2	PM14 transformed with pUC-CTX-2kan	TLC:RS1	No element	CTX-2kan-P	MG116025	2 × 10
PM14-U3	PM14 transformed with pUC-CTX-clakan	TLC:RS1	No element	CTX-clakan-P	MG116025	102
PM14-U4	PM14 transformed with pUC-CTX-O139kan	TLC:RS1	No element	CTX-O139kan-P	O395	2 × 102
					MG116025	10
PM9	V212-1 derivative	TLC:RS1:CTX-1:RS1	CTX-2kan:CTX-2	CTX-2kan-C2†	MG116025	103
PM22	B33 derivative	No TLC, No element	CTX-2kan:CTX-2	CTX-2kan C2	MG116025	5 × 103
O395-U1	O395 transformed with pUC-CTX-1kan	TLC:TrunCTX-cla:CTX-cla	CTX-cla	CTX-1kan-P	O395	5 × 104
A213-U1	A213 transformed with pUC-CTX-1kan	TLC	No element	CTX-1kan-P	O395	3 × 103

^*Transduction efficiency was calculated as the number of transductants per 6 × 10⁸ recipient cells per 1 mL of culture supernatant of the donor strain. The data represent the average of at least three independent experiments.

^†C denotes CTX phage produced from lysogenic phage integrated in chromosome 1 or 2.

^‡P denotes CTX phage produced from phage genome cloned in the recombinant plasmid.