Table 2.
Replication and transduction of CTX-cla and CTX-2
| Primary transduction | Secondary transduction | |||||
| Donor | pCTX produced from the donor | Transduction recipient strain | Transduction efficiency | Transductants (transduced pCTX) | Transduction recipient strain | Transduction efficiency |
| PM14-U2 | pCTX-2kan-P* | MG116025 | 2 × 10 | MG116025 (pCTX-2kan-P) | MG116025 | 2 × 104 |
| MG116025-toxTF139Y | 0 | |||||
| A213- toxTY139F | 3 × 103 | |||||
| IB4122 toxTY139F | 0 | |||||
| MG116025-toxTF139Y | 0 | |||||
| PM14-U3 | pCTX-clakan-P† | MG116025 | 1 × 102 | MG116025 (pCTX-clakan-P) | MG116025 | 3 × 104 |
| MG116025-toxTF139Y | 0 | |||||
| A213- toxTY139F | 2 × 104 | |||||
| IB4122- toxTY139F | 0 | |||||
| MG116025-toxTF139Y | 0 | |||||
| PM9 | pCTX-2kan-VL‡ | MG116025 | 103 | MG116025 (pCTX-2kan-VL) | MG116025 | 2 × 107 |
| MG116025-toxTF139Y | 0 | |||||
| A213- toxTY139F | 106 | |||||
| IB4122-toxTY139F | 1 × 103 | |||||
| MG116025-toxTF139Y | 0 | |||||
*
pCTX-2kan-P: pCTX-2kan produced from the plasmid pUC-CTX-2kan.
†
pCTX-clakan-P: pCTX-clakan produced from the plasmid pUC-CTX-clakan.
‡
pCTX-2kan-VL: pCTX-2kan produced from lysogenic CTX-2 in PM9, which is a derivative of strain V212-1.