. 2017 Feb 13;114(9):2241–2246. doi: 10.1073/pnas.1609579114

Table S1.

Thermodynamic properties of sfAFP derived from thermal denaturation at different thermal ramp rates

ΔT/Δt, °C/min	T_mid, °C	ΔH(T_mid), kcal·mol⁻¹	ΔS(T_mid), cal·mol⁻¹·K⁻¹	ΔH(4 °C),^* kcal·mol⁻¹	ΔS(4 °C),^* cal·mol⁻¹·K⁻¹	ΔG(4 °C),^* kcal·mol⁻¹
0.25	26.8 ± 0.2	56.1 ± 1.9	187 ± 6	46.5 ± 2.0	154 ± 7	3.89 ± 0.15
1	32.5 ± 0.2	57.3 ± 1.9	188 ± 6	45.6 ± 2.0	147 ± 7	4.78 ± 0.20
4	42.2 ± 0.2	54.1 ± 1.9	172 ± 6	38.6 ± 2.1	119 ± 7	5.57 ± 0.29
∞				38.1 ± 3.0^†	116 ± 12^†	5.92 ± 0.56^†

Fitting thermal melts used a fixed ΔC_p = 0.408 kcal·mol⁻¹·K⁻¹, which is calculated from ref. 23. See SI Methods for further details.

^{^*}

ΔS(4 °C) and ΔG(4 °C) are obtained by fitting the thermal denaturation data referenced to T_ref = 4 °C to minimize error propagation. ΔH(4 °C) is then calculated from ΔG(4 °C) + T_ref·ΔS(4 °C).

^{^†}

The infinite temperature ramp parameters are calculated by fitting ΔH(4 °C), ΔS(4 °C), and ΔG(4 °C) as a function of the scan rate, ΔT/Δt, assuming an exponential fit, that is,

Δ H (4 ° C, Δ T / Δ t) = Δ H (4 ° C, Δ T / Δ t \to \infty) + b \cdot e^{(Δ T / Δ t) / t 1} .

Additionally, ΔH and ΔS are fit globally with a shared “rate” t1 to facilitate fitting a dataset of only three scan rates.