Table S1.
ΔT/Δt, °C/min | Tmid, °C | ΔH(Tmid), kcal·mol−1 | ΔS(Tmid), cal·mol−1·K−1 | ΔH(4 °C),* kcal·mol−1 | ΔS(4 °C),* cal·mol−1·K−1 | ΔG(4 °C),* kcal·mol−1 |
0.25 | 26.8 ± 0.2 | 56.1 ± 1.9 | 187 ± 6 | 46.5 ± 2.0 | 154 ± 7 | 3.89 ± 0.15 |
1 | 32.5 ± 0.2 | 57.3 ± 1.9 | 188 ± 6 | 45.6 ± 2.0 | 147 ± 7 | 4.78 ± 0.20 |
4 | 42.2 ± 0.2 | 54.1 ± 1.9 | 172 ± 6 | 38.6 ± 2.1 | 119 ± 7 | 5.57 ± 0.29 |
∞ | 38.1 ± 3.0† | 116 ± 12† | 5.92 ± 0.56† |
Fitting thermal melts used a fixed ΔCp = 0.408 kcal·mol−1·K−1, which is calculated from ref. 23. See SI Methods for further details.
ΔS(4 °C) and ΔG(4 °C) are obtained by fitting the thermal denaturation data referenced to Tref = 4 °C to minimize error propagation. ΔH(4 °C) is then calculated from ΔG(4 °C) + Tref·ΔS(4 °C).