Table S1.
Biological pathways enriched for binding targets of (phosphorylated) CRYAB, yielded by WebGestalt analysis, using the Wikipathways database of biological pathways
| Rank | Pathway name | No. of targets/reference proteins | Adjusted P value* |
| Native CRYAB | |||
| 1 | Cytoplasmic Ribosomal Proteins | 24/78 | 4.32E-42 |
| 2 | mRNA Processing | 13/456 | 5.80E-09 |
| 3 | Prostaglandin Synthesis and Regulation | 3/31 | 0.0005 |
| 4 | Regulation of Actin Cytoskeleton | 4/160 | 0.0049 |
| 5 | Urea Cycle and Metabolism of Amino Groups | 2/22 | 0.0057 |
| 6 | Oxidative Phosphorylation | 2/60 | 0.0335 |
| P-Ser59 CRYAB | |||
| 1 | Cytoplasmic Ribosomal Proteins | 8/78 | 2.54E-10 |
| 2 | Glycolysis and Gluconeogenesis | 6/50 | 1.84E-08 |
| 3 | mRNA Processing | 11/456 | 6.70E-08 |
| 4 | TCA Cycle | 4/32 | 4.16E-06 |
| 5 | Electron Transport Chain | 5/101 | 1.47E-05 |
| 6 | Amino Acid Metabolism | 4/112 | 0.0004 |
| 7 | Striated Muscle Contraction | 3/44 | 0.0004 |
| 8 | T Cell Receptor Signaling Pathway | 4/143 | 0.0007 |
| 9 | Oxidative Phosphorylation | 3/60 | 0.0007 |
| 10 | Regulation of Actin Cytoskeleton | 4/160 | 0.0009 |
| 11 | Circadian Exercise | 2/49 | 0.0084 |
| 12 | FAS Pathway and Stress Induction of HSP Regulation | 2/50 | 0.0084 |
| 13 | MAPK Signaling Pathway | 3/165 | 0.0089 |
| P-Ser45 CRYAB | |||
| 1 | Cytoplasmic Ribosomal Proteins | 8/78 | 5.15E-09 |
| 2 | Glycolysis and Gluconeogenesis | 6/50 | 1.98E-07 |
| 3 | DNA Replication | 5/41 | 2.03E-06 |
| 4 | Proteasome Degradation | 5/59 | 9.69E-06 |
| 5 | Cell Cycle | 5/95 | 8.15E-05 |
| 6 | TNF-α NF-κB Signaling Pathway | 6/213 | 0.0003 |
| 7 | G1 to S Cell Cycle Control | 4/65 | 0.0003 |
| 8 | Myometrial Relaxation and Contraction Pathways | 5/158 | 0.0006 |
| 9 | Striated Muscle Contraction | 3/44 | 0.0011 |
| 10 | mRNA Processing | 7/456 | 0.002 |
| 11 | PluriNetWork | 5/289 | 0.0064 |
| 12 | Circadian Exercise | 2/49 | 0.0221 |
| 13 | Wnt Signaling Pathway NetPath | 3/141 | 0.0221 |
| 14 | Calcium Regulation in the Cardiac Cell | 3/152 | 0.0252 |
| 15 | Oxidative Phosphorylation | 2/60 | 0.0282 |
| 16 | IL-5 Signaling Pathway | 2/80 | 0.0453 |
| P-Ser19 CRYAB | |||
| 1 | Cytoplasmic Ribosomal Proteins | 31/78 | 4.19E-52 |
| 2 | mRNA Processing | 23/456 | 9.76E-17 |
| 3 | Electron Transport Chain | 5/101 | 0.0005 |
| 4 | Oxidative Phosphorylation | 3/60 | 0.0104 |
Listed are pathways that are enriched for binding targets of (i) native, (ii) phospho-Ser-59, (iii) phospho-Ser-45, and (iv) phospho-Ser-19 CRYAB. Most highly enriched pathways (compared with other CRYAB phosphorylation states) are depicted in bold, and pathways uniquely enriched for targets of a specific phosphorylation state are depicted in italics.
*
P value determined by hypergeometric testing, after Benjamini–Hochberg adjustment for multiple testing.