Table S2.

Ca²⁺ binding by CABP2-wt and CABP2-ΔEF3/4: Isothermal titration calorimetry data for Ca²⁺ binding to wild-type CABP2 and the missense mutant previously reported lacking C-terminal binding sites

	CABP2-wt		CABP2-EFΔ3/4
ITC	Site 1	Site 2	Site 1
Kd, μM	50.14 ± 1.18	141.57 ± 4.29	38.40 ± 4.96
ΔH, kcal/mol	1.46 ± 0.08	1.32 ± 0.05	1.52 ± 0.12
ΔS, kcal/mol	6.90 ± 0.01	6.27 ± 0.06	7.21 ± 0.05

Note the low Ca²⁺ binding affinity of CABP2-wt and the missing Ca²⁺ binding site in CABP2-ΔEF3/4. K_d, dissociation constant; ΔH, change of enthalpy; ΔS, change in entropy.