Fig. S4.

Polyreactivity of 9H4 against synthetic haptens. (A) Immunofluorescence staining of 2% PFA fixed, and Triton X-100 permeabilized untreated and IR-treated NDF cells stained with IgM 9H4 clone (red). DNA was stained with DAPI (blue). (Scale bar, 5 μm.) (B) Polyreactivity of IgM 9H4 or whole-molecule IgM. Binding value was assessed for each antibody by testing their binding to the indicated BSA-conjugated haptens and dsDNA or ssDNA. Values (arbitrary units) and SDs were calculated from triplicates. (C) Reactivity of anti-PC antibody (E06) or whole-molecule IgM to BSA or BSA-conjugated PC. Values (arbitrary units) and SDs were calculated from triplicates.