Fig. S6.

MDA-modified vimentin detected in oncogene-induced senescent and late passage NDF cells. (A) Isolated membrane lysates from IR-treated NDFs were immunoprecipitated with a commercial antivimentin antibody. Immunocomplexes were probed for vimentin, MDA, CML, and PC (E06 antibody), respectively. (B) Quantification of SA-β-Gal⁺ NDF cells transduced with Tet-inducible HRas G12V in the presence or absence of doxycycline (14-d postinduction). Averages and SDs are from three independent experiments. An unpaired Student’s t test was used for statistical analysis. (C) Quantification of SA-β-Gal⁺ NDF cells at early (8 PD) and late (64 PD) passages. Averages and SDs are from three independent experiments. An unpaired Student’s t test was used for statistical analysis. (D) Sequence homology of vimentin surrounding the single cysteine residue.