Fig. 5.

NPY-induced cellular proliferation is further enhanced by inhibition of miR-375 inhibitor. T84 cells were treated with NPY, and miRNA was extracted using Qiagen miRNAesy kit. The expression of miR-375 was assessed by real-time PCR. In a separate experiment, T84 cells were transfected with negative control miR-375 or miR-375 inhibitor (30 nM) using lipofectamine RNAiMAX, followed by treatment with NPY (0.1 µM) for 24 h. The lysates were probed for proliferation cell cycle regulators and apoptotic markers by Western blotting. A: miR-375 expression in control and NPY treated cells. B: Western blots for c-Myc p21, and Cyclin D1. C: the corresponding histograms. Values are means ± SE; n = 4. *P < 0.05, **P < 0.01, ***P < 0.001.