Fig. 1.

Active Notch signaling occurs in the mouse corpus epithelium. A: GFP immunostaining of an isolated corpus gland from the CBF:H2B-Venus Notch reporter mouse. Multiple GFP⁺ nuclei are present (arrows), indicating epithelial cells undergoing active Notch signaling. B and C: qRT-PCR analysis of Notch1–4 receptors in full-thickness corpus tissue (B) or corpus glands (C). ND, not detected. Data are presented as means ± SE (n = 4–5 mice/group). D and E: coimmunostaining of NOTCH1 (green) and EdU (red; D) or NOTCH2 (green) and EdU (red; E). White dashed boxes are magnified in the insets. Arrowhead indicates NOTCH2⁺ epithelial cells at the base of the corpus glands. Scale bars: 50 μm.