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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Exp Hematol. 2016 Oct 15;46:83–95.e6. doi: 10.1016/j.exphem.2016.10.002

Fig 5. IL-6 signaling functions downstream of Hif1α in AGM HSPC regulation.

Fig 5

(A) RT-qPCR analysis demonstrated that expression of il6 and its receptor il6R are increased by CoCl2 exposure (*p<0.05, **p<0.01, one-tailed t-test, n≥3).

(B) Morpholino knockdown of il6 diminished the ability of CoCl2 to stimulate runx1;cmyb expression in the VDA over that seen in controls as determined by WISH analysis at 36hpf.

(C) Qualitative phenotypic distribution of embryos from panel 5B (n≥20/condition × 3 replicate clutches).

(D) FACS analysis confirmed that CoCl2 was unable to enhance the number of CD41+ HSPCs in the presence of il6 knockdown (*p<0.05, one-tailed t-test, n≥4).

(E) Injection of dnhif1a mRNA decreased runx1;cmyb expression in the VDA at 36hpf, which could be partially ameliorated by overexpression of il6.

(F) Qualitative phenotypic distribution of embryos from panel 5E (n≥20/condition × 3 replicate clutches.