Fig. 3.

High-resolution mapping of DNA supercoiling reveals hundreds of supercoiling domains in the human chromosome (HSA) 11. a Microarray data of biotin-tagged psoralen bTMP binding across HSA 11 to assay the level of DNA supercoiling. α-Amanitin is used to inhibit transcription. Supercoiling domains are categorized as underwound, overwound, or stable regions. b The distributions of supercoiling domains. c Model of the large-scale chromatin structures. An overwound domain corresponds to transcriptionally inactive chromatin and is compact over a large scale, whereas an underwound domain corresponds to a transcriptionally active region and a decompacted chromatin structure. Adapted from Naughton et al. (2013) with permission