Figure 3.

Essential role of JA signaling in the plant response to reoxygenation. A, phenotypes of wild type (WT), jar1-1, and coi1-2 before submergence (Air) and after 48 h dark submergence (Submergence) followed by reoxygenation 4 d (Reoxygenation). B, Survival rates (top) and dry weights (bottom) of WT, jar1-1, and coi1-2 after 2 d dark submergence followed by a 4 d reoxygenation period. The data are means ± sd (n = 3 independent experiments). For each experiment, at least 15 plants were used for each genotype. Asterisks indicate significant differences from the untreated or WT control (**P < 0.01 by Student’s t test). C, Analysis of JAZ1-GUS protein stability before submergence (Air), after dark or light submergence (DS or LS), and during reoxygenation (R1). Ten-day-old transgenic lines expressing JAZ1-GUS in WT and coi1-1 mutant backgrounds were untreated or treated with DS or LS for 24 h followed by a 24 h reoxygenation period. The seedlings were collected and were subsequently stained with X-Gluc.