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. 2017 Jan 26;173(3):1606–1616. doi: 10.1104/pp.16.01450

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Figure 2. — Phosphorylation of recombinant p26 by CPKs in vitro. A and B, Incorporation of ³²P into p26 after in vitro kinase assays using recombinant AtCPK34, CKII, or PKA with recombinant p26a (A) and p26b (B). Top gels are autoradiographs showing ³²P incorporation (total pmol), and bottom gels are western blots to show equal loading of protein. C and D, Incorporation of ³²P into p26a (C) and p26b (D) during in vitro phosphorylation using PrCPK17/34, PrCPK14, PrCPK6/26, or AtCPK34. Values are means ± se (n = 3).