Nucleotide sequence of NlEG1 and its deduced amino acid sequence. The arrow indicates the signal peptide cleavage site. Predicted N-glycosylation and O-glycosylation sites are underlined in black and highlighted in gray, respectively. The square region denotes the proton-donor catalytic region. The catalytic nucleophile, probable secondary nucleophile, and proton acceptor (Glu) of the conserved catalytic domain for GHF 9 members are represented as a black diamond, a gray diamond, and a black circle, respectively. Two GHF 9 signature motifs are underlined in gray.