Figure 3.

NlEG1 in rice and its silencing efficiency by RNAi. A, Detection of protein NlEG1 in rice infested by BPH nymphs. Protein samples for western-blot analysis were as follows: extract from the salivary glands (lane 1) and extracts from rice plants that were infested by nymphs (lane 2) or kept noninfested (lane 3). The black arrows represent the target band. B and C, Mean transcript levels + se (n = 3) of NlEG1 in whole bodies on different days (B) and in different tissues of newly emerged brachypterous female adults 3 d (C) after they (fifth-instar nymphs) had been injected with dsRNA of NlEG1 (dsNlEG1) or GFP (dsGFP) or kept noninjected (control). Sg, Salivary gland; Mg, midgut; Ov, ovary; Fb, fat body. D, Mean endo-β-1,4-glucanase activities + se (n = 4) in the whole body 3 d after the insects received the same treatments as above. The inset shows western-blot analysis for NlEG1 in the salivary glands of BPH that received the same treatments as above. Letters indicate significant differences among different treatments (P < 0.05, Duncan’s multiple range test).