Figure 3.

Localization of natively pollen-expressed EXO70 isoforms in growing tobacco pollen tubes. Selected YFP-tagged tobacco EXO70 isoforms were transiently expressed in tobacco pollen tubes, and their subcellular localization was examined by spinning disk confocal microscopy. Growing pollen tubes with low expression levels of the transgene are shown. The images shown are representative for 20 or more transformed pollen tubes observed in at least two independent experiments for NtEXO70F, NtEXO70G1a, and NtEXO70H1-2 and for 30 or more transformed pollen tubes in three or more independent experiments for the rest of the isoforms. Arrowheads mark small areas of membrane localization for NtEXO70A1a and concentration of signal in the corresponding area for NtEXO70A2. In the case of NtEXO70H1-2, the contour of the pollen tube is marked because of negligent cytoplasmic signal due to nuclear accumulation of the signal. Bar = 10 µm.