Figure 7.
Colocalization of NtEXO70A1a with lipid markers in growing tobacco pollen tubes. NtEXO70A1a was transiently coexpressed together with the PA marker mRFP:2Spo20p-PABD (left) and the PIP2 marker mRFP:2PHPLCδ1 (middle), and the subcellular localization of the constructs was examined by spinning disk confocal microscopy. Images of individual channels are represented using a color intensity code in order to display local enrichment of the YFP/mRFP signal. In the overlays, YFP is represented by green, mRFP by magenta, and white indicates the overlapping signal. Red and blue arrowheads mark the onset and end of the particular membrane signal, measured as the equatorial distance from the pollen tube apex (right; n ≥ 13). The onset value for mRFP:2PHPLCδ1 was set to zero because it always covers the very tip of the pollen tube PM. Bar = 10 µm.